Validity of the jack-knife technique for analysing enzyme kinetic data.
نویسندگان
چکیده
An observation by Duggleby [Biochem. J. (1979) 181, 255-256] that estimates of kinetic parameters by the jack-knife technique [Cornish-Bowden & Wong (1978) Biochem. J. 175, 969--976] are sometimes outside the range of estimates from which they are calculated has been examined. No significant correlation has been found between the occurrence of this behaviour and the actual quality of the estimates.
منابع مشابه
Kinetics and Isotherm Studies of the Immobilized Lipase on Chitosan Support
The kinetics and isotherm studies of the immobilized lipase and the mechanism of immobilization on chitosan beads and activated chitosan beads with glutaraldehyde were investigated. The effect of glutaraldehyde on porosity of chitosan was evaluated by FESEM analysis. It was observed that the porosity of the carrier which has activated by glutaraldehyde was substantially increased. The validity ...
متن کاملThe Kinetics of Enzyme Mixtures
Even purified enzyme preparations are often heterogeneous. For example, preparations of aspartate aminotransferase or cytochrome oxidase can consist of several different forms of the enzyme. For this reason we consider how different the kinetics of the reactions catalysed by a mixture of forms of an enzyme must be to provide some indication of the characteristics of the species present. Based...
متن کاملTrajectory Tracking Weeled Mobile Robot Using Backstepping Method with Connection off Axle Trailer
The connection of the tractor to the inactive trailer or motor vehicle causes a motion control problem when turning in the screw, forward or backward movements and high speeds. This is due to the inactive trailer being controlled by the tracking using a physical link that is not affected by the movement. Trailers usually take tracks under these conditions. This phenomenon is called Jack Knife. ...
متن کاملKinetic Modeling of Enzymatic Hydrolysis of Pretreated Sorghum Bicolor and Rice Husk
In this study, the hydrolysis of pretreated sorghum stem and rice husk was investigated at various initial enzyme concentrations and substrate loadings. The slowdown in enzymatic hydrolysis of lignocellulosic materials with conversion has often been attributed to decreasing the activity of enzyme. A kinetic model was developed and expressed mathematically based on enzyme deactivation for enzyma...
متن کاملThe Correlation of Biological Activity and Chemical Structure of Quinolizidinyl Derivatives as Inhibitor of Alzheimer’s Disease with Linear and Non-linear Models
In this research,QSAR study has been carried out on quinolizidinyl derivatives as potent inhibitors of Acetyl and Butyrylcholin esterase in Alzheimer’s disease. Despite significant research efforts in both industry and academia, there are currently no diseases modifying therapies available to treat this illness. Significant evidence suggests that the pathology of AD is linked to generation of β...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Biochemical journal
دوره 185 2 شماره
صفحات -
تاریخ انتشار 1980